The three methods for inoculation of high yield of shiitake mushrooms

Dense inoculation involves increasing the fungal coverage, which creates a larger germination area for bacteria. This allows the mycelium to penetrate eucalyptus wood more quickly within a shorter time frame. The exchange of gases between the eucalyptus and the environment primarily occurs through water absorption and evaporation. Alternating wet and dry cycles help provide sufficient oxygen to the interior of the wood, enabling the hyphae to grow deeper into the eucalyptus. This process not only accelerates the maturation of the wood but also reduces the chances of bacterial infection. Deep inoculation refers to planting the fungal spores at a greater depth. This method is more effective in promoting drought resistance and minimizes the impact of temperature fluctuations on the seed block located at the bottom of the hole. Under stable temperature and humidity conditions, the mycelium can germinate rapidly, significantly improving survival rates. By deepening the hole, the tangential direction of the wood is extended, allowing the hyphae to access more nutrients, which ultimately enhances the overall yield. Early inoculation takes place under low-temperature conditions, when the concentration of spores in the air is lower and their activity is reduced, making it less likely for them to develop into mycelium. However, shiitake mushroom mycelium can thrive in such conditions due to its strong resistance and vigorous growth. This results in higher survival rates, making early inoculation an effective strategy for successful cultivation.

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