After the flowering season ends, it's essential to remove any remaining spent blossoms. At the same time, trim back overlapping or crossing branches, as well as any dead or diseased limbs. This helps prevent unnecessary nutrient depletion and improves air circulation within the plant, reducing the risk of pests and diseases like scale insects or black spot. Proper pruning also encourages healthier, more vigorous growth.
Camellias consume a lot of nutrients during the blooming period, from bud formation to flower development. To restore the plant’s vitality quickly, regular fertilization is necessary. After flowering, apply a diluted liquid fertilizer every other week. You can make this by fermenting materials like bean cake, hoof meal, fish bone powder, and black leeks. Once decomposed, strain the mixture and dilute it with water before use. Avoid using concentrated fertilizers or applying them directly to the roots, as this can cause root burn. Additionally, you can apply a foliar spray of 0.2% potassium dihydrogen phosphate every 10 days, up to five times, to promote thick new growth and enhance bud formation for the next season.
Watering should be done carefully. From mid-April onward, when the weather tends to be dry and humidity is low, it's important to maintain proper moisture levels. Water the plants regularly, and mist the leaves once or twice daily to increase local humidity, which supports better overall growth.
If your camellia is in a pot, consider repotting it when the roots have filled the current container. Choose a larger pot and add slow-release fertilizers like hoof meal or hair as a base. After repotting, water thoroughly and place the plant in a cool, shaded area for about 20 days before resuming regular care. This allows the plant to settle in and recover before being exposed to full sunlight or intense watering.
DNA/RNA Purification Kit
The RNA purification kit is used to purify and recover RNA molecules transcribed in vitro and total RNA extracted from various materials, which can effectively remove contaminating impurities in RNA samples. The recovery rate of this product can reach 80%, and the OD260/OD280 ratio of the obtained RNA is generally about 2.0, which can be directly used in subsequent sensitive experiments (such as microarray analysis, fluorescence RT-PCR, etc.). With the deepening of transcriptomics, the complexity of RNA types, expression regulation and functions is far beyond our imagination.
Removing ribosomal RNAs that account for more than 80% helps to focus sequencing on less abundant but informative RNAs. At present, the removal of rRNA is mainly through the combined use of probe and RNase H. The processed RNA will be mixed with many digestion products, enzymes and ions, which is not conducive to the subsequent construction of RNA library. Take the Columnar RNA Purification Kit as an example. Trizol is a ready-to-use reagent that can be used to purify total RNA from tissues and cells. This is a single-phase solution of phenol and guanidine isothiocyanate that facilitates lysis of tissues and cells, inhibiting RNases to maintain RNA integrity.
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