Difficulties and Countermeasures in Cultivation of Flammulina velutipes

Difficulties and Countermeasures in Cultivation of Flammulina velutipes First, white flocculent aerial hyphae appear on the surface of the bag material. Reasons: 1. Bacteria bag water content is low, moisturizing bacteria stage lack of air humidity. 2. After the bacteria were inoculated, the indoor concentration of carbon dioxide in the shoots was high and the ventilation was insufficient, which prolonged the time from vegetative growth of mycelium to reproductive growth. Countermeasures: 1. Add the culture medium after the second fermentation after the Fertilizer Type 1 Ferment Starter to adjust the water content. 2. Increase the relative humidity of air in the moisturizing and germination stage to prevent overgrown aerial mycelium and form pellicles. In addition, we must also do a good job of moisturizing and ventilation indoors during the budding stage.
Second, after 3 days of fungal spores, no hyphae were recovered. Cause: The air in the environment of the reminder bud room is over-circulated, and the bag material surface cover is not covered. Countermeasures: Concentrate the treatment, and use a cold boiled water to lightly spray 2 to 3 times in 2 days to make the material surface wet, and then cover it after spraying for 10 minutes to keep the air between the cover and the material surface with high humidity.
Third, the bag material surface was black and moist. Reasons: 1. Spray raw water directly on the material surface. 2. The film covers the material surface for a long time, causing the condensate on the film to drip back, causing the hypha to shrink and the material surface to become black. Countermeasures: Dig up the blackened culture material with a sterile knife and rehydrate the bacteria.
Fourth, the mushroom bud discolored dead. Cause: The small water droplets on the material surface of the induction mushrooming stage failed to evaporate in time, causing the original base of the mushroom bud to be submerged in water and suffocate. Countermeasures: When there are small drops of water on the waiting surface, gradually increase the amount of ventilation in the room so that these small drops of water evaporate as soon as possible. The color of waterdrops is light [shielded], and it is normal when clear. If it is brown and cloudy, it indicates that the bacteria species have been infected by bacteria.
Fifth, the original base is densely packed with effective mushrooms. Reasons: Mainly caused by the asynchronous development of Flammulina velutipes. In order to suppress the opening of the umbrella, one-sided increase in the concentration of carbon dioxide, prematurely pull up the plastic film folded under the bag, or bagging too early, so that most of the mushroom bud because of not get enough oxygen to suffocate. Countermeasures: Different ventilation methods should be adopted according to different seasons and different cultivation environments. In the suppression stage, the ventilation of the room should be increased to make the mushroom buds grow taller. In the spring and summer season, the amount of indoor air circulation should be increased in the rainy season to maintain the relative humidity at 80% to 85%. Elevating fans blow air to ensure that every layer of air on the cultivation frame can fully flow.
6. There is a "swiss mushroom" with mushroom cap connected and a mushroom stalk flat. Causes: Insufficient ventilation in the mushroom house, and hyphae did not reach the standard of physiological maturity, the medium was too dry, and the strains were aging. Countermeasures: Use a simple CO2 meter to monitor the carbon dioxide content in different developmental stages of the fruiting body of the mushroom house. During the development of the fruiting body, it should receive light for 2 hours per day and be performed several times.
Seven, mushroom buds are of different thicknesses. Cause: The bud suppression failed. Countermeasures: In the process of budding, it is necessary to frequently clear the buds and remove the particularly strong mushroom buds by hand.
Eight, "water mushroom" appears. Cause: The relative humidity of the air in the mushroom house is high. Countermeasures: Reduce the humidity of the air in the mushroom house as much as possible. Fans are blown with a reciprocating vertical fan or a shaking head fan to enhance air convection. Adjust the proportion of good intake and exhaust volume. The fresh air to be replenished should be pre-cooled and dehumidified first, and air flow should be increased two days before harvest.
Nine, open the umbrella during the development of mushroom buds. Reasons: 1. The quality of bacteria bags. If the mycelium is loose and the material is not tight, even if it is a mushroom, it is easy to open the umbrella. 2. Temperature, humidity, oxygen, and illumination are not coordinated during cultivation and management. Countermeasures: During the cultivation of bacteria bags, air convection should be strengthened to prevent the heat generated during the germination stage from being lost. Also, check whether the moisture content of the culture material is lower than 50% or higher than 70%, and check whether the sterilization of the fungus bag is complete. [ Is shielded] whether the species is recessively contaminated? To identify whether [shielded] strains are contaminated, [shielded] strains and uninoculated bacteria bags can be returned to PDA medium under sterile conditions.

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