Establishment of related functional database system for rice functional gene research

For the first time, Chinese researchers have successfully established a rice functional gene database system, which laid the foundation for the prediction and discovery of new functional genes.
According to reports, this research project titled “Research on Functional Genes Related to Important Agronomic Characters such as Rice High-yield” is a major program of functional genes and bio-chips in the biological and modern agricultural technology field of the 863 Program of the Ministry of Science and Technology; Under the key support of the Bureau of Experts and the Beijing Foreign Experts Bureau, Beijing Kaiming Agricultural Biotechnology Co., Ltd., Beijing Huada Genetic Research Center, Beijing-Yale Joint Research Center (Peking University), and Genetics and Developmental Biology of the Chinese Academy of Sciences The institute's R&D personnel, such as the Chinese Academy of Agricultural Sciences and other units, after four years of hard work, jointly completed and passed the acceptance by the Ministry of Science and Technology.
The researchers said that the establishment of a related database system for functional gene research in rice and the establishment and development of a website system (http://rise.genomics.org.cn) that has an open search-oriented and application-oriented function for efficient search engines provide rice research The world's most complete raw rice sequence data and various research methods. Comparing the comparative genomes of different rice cultivars such as super hybrid rice parent 9311 (father), Pei'ai 64S (female), Liangyoupeijiu (hybrid combination), Zhenyu 97A (female), Minghui 63 (father), and Nipponbare Scientific analysis will reveal the molecular mechanism of gene doubling in genome evolution, find the molecular basis of rice hybridization, and the molecular mechanism of hybrid superiority. It will be possible to discover new genes related to agronomic traits such as hybrid superiority, and can also be used for the same grasses. Gene research on corn, wheat, sorghum and other crops provides a reference.

Goji Polysaccharides is a rare plant polysaccharides containing protein, which have side chains of polysaccharide composed by six carbon sugars (rhamnose, arabinose, xylose, mannose, galactose, glucose) and main chains of protein composed by 18 kinds of amino acids. The glycoproteins with biological activity is a high-strength immune enhancer.

Wolfberry products with stable quality are all natural, safe, sanitary and fast absorption.Lycium barbarum polysaccharide extraction and purification is divided into two steps. First, the extraction of Chinese wolfberry crude polysaccharides, the second is purified Lycium barbarum polysaccharide. Crude polysaccharides extraction process is as follows: Weigh dried crushed medlar, petroleum ether: acetone (1: 1) reflux degreasing, the solvent was filtered off, the residue was air-dried 80% ethanol off monosaccharide and oligosaccharides. 
The polysaccharide is extracted by water after dissolving the residues such as oligosaccharides in water at 90-100 DEG C, the extract is concentrated under reduced pressure, the polysaccharides are precipitated by ethanol, the precipitates are washed with anhydrous ethanol and acetone, and the lycium polysaccharides are dried under vacuum. The crude polysaccharide is purified by passing the Lycium barbarum polysaccharide through a column of DEAE cellulose with different concentrations of NaCl as an eluent, concentrating the eluates with different salt concentrations respectively under reduced pressure, desalting them by dialysis, and then lyophilizing them differently Grape Lycium glycoprotein.

Modern medical research suggests that wolfberry polysaccharides can improve human immunity and have functions as anti-cancer, cancer treatment, hematopoiesis, liver protection and hypotensive, free radicals removal , anti-fatigue, anti-radiation, and cell activity improvement; Goji polysaccharides can control cells division& differentiation, and regulate cell growth & aging, which is widely used in the pharmaceutical, health food and other fields.

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