Artificial domestication breeding of wild mountain wild fish

Palea steindachneri is a national second-class animal. Since the resources of wild mountain wild turtles have been reduced year by year, it is necessary to domesticate and reproduce the mountain wild turtles.
1?Materials and methods
1.1? Domestication
1.1.1 Domesticated Ponds The domesticated ponds have an area of ​​2,000 to 3,333 m2 and a water depth of 1.5m. Aquatic plants are planted in shallow waters near the pond. The environment is quiet. There are "T' type escape walls around the pond and the pond faces the south side of the river. The 20m2 spawning ground will be constructed on the sandfield with rain-proof shaded stone-cotton tile sheds. The middle and east of the pond will be separated by 2m to build a wooden drying platform and food table. The water surface will be planted with Eichhornia crassipes (the total area is 1/5 of the water surface).
1.1.2? Mountain Rui source?
Choose from the mountain or the market to buy wild mountain wild rice with a weight of 1 ~ 2kg, no injury, disease-free, robust and inferior, bright skin, thick skirt with a thick trailing edge of the back nail, and agile action.
1.1.3 feeds and domesticated feeds use commercially available full-price feeds, animal feeds use fresh fish, snails and shrimps; animal feeds are soaked in 5% salt water for 15 to 20 minutes. Wash it with clean water afterwards. Taming the food using a small number of multi-point method, on the table 5cm away from the waterside animal feed to attract food, 18:00 at 18:00 daily, the first 2d morning to remove residual food. By the 8th day, Sanriru began to crawl on the food platform. After the 15th day, the domestication process was basically over. Shan Ruiji had adapted to the food on the food table. At this time, he went into normal feeding and fed it every day. The amount of 3% to 5% of body weight was fed in the morning and evening, and the feed was gradually fed at full price, supplemented with animal feed, and a small amount of green feed was added.
1.1.4? Daily management and disease prevention?
In accordance with the "Four Sets" principle of feeding and management, observe more and carefully make all relevant records. Domestic ponds are regularly filled with fresh water to remove residual food in a timely manner to keep the water fresh. Strengthen diarrhea prevention and control, every 20 days with conventional disinfectants such as dibromohydantoin, complex iodine or quicklime 10 ~ 15kg/667m2 into lime milk Quanchiposa uniform disinfected once, while feeding antibiotic baits, once a day, even Hello 3d.
1.2 breeding and hatching
1.2.1? Intimate cultivation
In November, the strong-selected Shanrui (♀:♂=3:1) from the domestication ponds was used as an artificially-breeding inbred to move into a nursery pond for cultivation. Intimate breeding ponds have plastic film insulation sheds. The cultivation pond area is 260m2, the brickwork structure, inside the block, the bottom of the pool is placed with 20cm thick yellow mud, the water depth is 1m, and there is a food table and sun drying platform. A 20m2 spawning sand field is built at one end of the pool. Keep the temperature of the water in the boiler at 25°C or so, feed it with animal feed, and feed the full price with cockroach feed and green feed. Strengthen water quality management and disease prevention, and apply photosynthetic bacteria once a month.
1.2.2?Mating Spawning Under artificially controlled temperature conditions, Shanruqin can mate at a water temperature of 22°C to 25°C, and 15 to 20 days after mating, the female baboons begin to spawn. The spawning takes place in the middle of the night. The female cubs climb onto the battlefield to find a good position, dig nests for spawning, and cover up after laying eggs.
1.2.3 Artificial hatching Incubator Room 10~15m2, covered with heat-insulating foam board on four walls and top surface, building a small ditch along the wall and injecting water and a little fine sand; hanging a UV lamp for disinfection and sterilization, and using electric furnace The temperature is controlled by a thermostat to control the room temperature.
After 3 days of pro-spawning, the fertilized eggs are collected in an incubator and incubated in boxes. The method is: first in the bottom of the wooden box 50cm40cm15cm supplemented with 3cm thick hatching sand, then according to the egg spacing 1cm, the animal pole (white point) upwards to release a good fertilized egg, and then cover the hatching sand 10cm, placed on the iron frame, Manual temperature control for incubation. The hatching sand must be screened, contaminated, disinfected and exposed to sunlight to adjust the humidity of the sand (the humidity is 4% to 5%, that is, it is appropriate to disperse it by hand after loosening it by hand). After about 60 days of hatching, the juvenile can hatch out.
During the incubation process, strictly control the room temperature at 302°C, control the indoor air humidity at 80% to 85%, and maintain the humidity of hatching sand.
2 results
2.1 Domestication Results Domestication trials A total of 964 wild mountain raccoons were purchased, including 703 females and 261 males, with a male to female ratio of 2.7:1. After more than two years of domestication trials, the number of acceptances for Ruitang in Cangtang Mountain was 800, and the survival rate was 83.0%. Among them, 623 were females in the pond, and the survival rate was 88?6%; 187 of the males in the pond had a survival rate of 71.6%. ?
2.2? Egg production rate?
It has been observed that in April and May (Guangzhou-Guangxi area), the peak period of spawning of Sanrethi, and the spawning is basically completed by the end of June. Shan Ruiqi only spawns eggs once a year. In the first year of the trial, females spawned 218 nests, and 309 females entered the nursery pool. The egg production rate was 70.6%.
2.3? Fertilization rate?
According to records, the number of spawnings of Sanrethi was 5 to 20 pieces/fowl, and the majority was 10 to 18 pieces/flock, with an average of 15.6 pieces/flock. A total of 3,480 eggs were collected from 218 litters, of which 1,378 were fertilized eggs and the fertilization rate was 39.6%. In terms of oviposition per litter, the minimum fertility rate was 22.3% and the highest fertilization rate was 46.8%. ?
2.4? Hatching rate?
The 1378 fertilized eggs hatched in the hatchery were hatched after about 60 days and 12,25 hatchlings were hatched. The hatching rate was 88.9%. Of these, 2 were malformed, accounting for 0.2%. ?
3 Discussion
3.1? Morphological characteristics Through observation, it is concluded that the external morphological features of S. sinensis are: flat and round in shape, with a wide and thick skirt at the back of the body, and a row of distinct, regular granular scorpions on the leading edge of the carapace. There are also scattered, distinctly granular pods with grayish white ventral surface and more black cloud spots. The base of the neck has a group of verrucous protrusions and rough skin.
3.2? Ecological characteristics Through observation, investigation, and research, it is believed that Shan Ruixi prefers to live in a stream. He particularly likes to live in streams with a trickle and quiet environment. It mainly eats animal foods such as snails, clams, fish and shrimps and insects. , supplementary food plant foods. The suitable water temperature for growth is 20°C to 32°C, and the optimal water temperature for growth is 25°C to 32°C. When the water temperature is below 12°C, hibernation begins. During hibernation, activities and feeding are performed as long as the water temperature rises above 18°C. Shan Ruiqi is docile, has a timid nature, and is slow in action. His back and spawning are all closer to the water.
3.3? Domestication situation?
From the results of domestication, the survival rate of males is lower than that of females. The analysis suggests that the main reason may be that males are sexually aggressive and injured, causing various diseases to die.
In the first year of the 3.4 spawning test, the spawning rate reached 70.6%, which is relatively normal. Some females may not have reached the maturity requirement.
3.5. Fertility rate The low fertilization rate may be related to the fact that the cultivation environment did not meet the requirements of the Sanreshi ecological environment, or the nutrition of the feed did not meet the requirements for innate breeding. For further study.
3.6? Hatching rate According to the hatching of Chinese sturgeon, the hatchling can be hatched after about 60 days when the maximum particle size of hatching sand is 0.5mm, the relative humidity is 4% to 5%, and the average temperature is 30°C. The hatching rate was 88.9%. I believe that the normal level of incubation has been reached.
As this is the first year of the trial, many aspects of domestication and breeding are still being explored. We will sum up experience and further explore the artificial domestication and breeding techniques of wild mountain wild turtles to provide technical support for large-scale domestication and reproduction.

Bleach Clean-up Enzyme is developed to remove residual hydrogen peroxide.Usually the cotton fabric bleached with hydrogen peroxide, the bleaching chemicals must be removed before dyeing in order to avoid the reaction between the bleaching agent and the dye. The traditional way to remove bleach is to rinse the fabric several times with water. Alternatively, you can use a mild reducing agent to clean the bleach. In addition, a large amount of water is needed in rinsing which wash out a large amount of wastewater.

Conzyme CAT200 is an extremely high concentrated liquid catalase preparation. It is specially developed to remove residual hydrogen peroxide and improve the last preparation prior to dyeing; it can largely reduce hazardous chemicals usage and save water.

Benefits:

Complete removal of residual H2O2 in preparation for dyeing
Wide pH range, convenient in using
No damage of fabric
Reduce processing time
Reduce water consumption and effluent volume

Environmental-friendly & bio-degradation


Enzyme preparations belong to protein, which may induce sensitization and cause anaphylaxis in sensitized individuals. Long-term exposure may cause minor irritation for skin, eyes or nasal mucosa, so any direct contiguity with human body should be avoided. If irritation or allergic response for skin or eyes develops, consult a doctor.

Bleach Clean-up Enzymes

Bleach Clean-Up Enzymes,Catalase Enzyme,Enzyme For Peroxide Clean Up,Bleach Inhibitor

Sunson Industry Group Co., Ltd , http://www.sunsonchinaenzymes.com