Stingray is also known as squid and eel, and the name is also known as fangs. Its delicate meat, delicious taste, is a superior quality aquatic products, more and more popular with consumers. In recent years, the experimental group of the Fisheries Technology Extension Station in Liangshan County of Shandong Province has conducted continuous research and exploration and obtained good results. The techniques for artificial breeding and seedling cultivation of non-pollution stingray are described as follows: artificial propagation
1. The choice of broodstock. In the breeding season from May to June, females were selected to weigh between 100 grams and 125 grams, and the abdomen expanded. Obviously, the ovary was clearly visible on the abdomen. Inverted ovarian flow was observed. The gonads expanded, and they were wide and round in red. Hand touch the fish's belly, there is a feeling of soft and elastic. Male fish weighing 125 grams to 175 grams and dark yellow body color, long and sharp genital protrusion, protruding about 0.5 cm or more, the reproductive hole was pink. The male to female ratio is 1:1.5.
2. Fertility production. Induction production is carried out in a flowing cement tank. The water quality of the source meets the requirements of GB11607 and the requirements for pond water Nr5051. The urine production pond area is 6 to 10 square meters, the water depth is 0.6 to 0.8 meters, and the water temperature is 24 to 26 degrees Celsius. Oxygen production drugs use luteinizing hormone-releasing hormone analogues (LRH-A
2) Mix with human chorionic gonadotropin (HCG) at a dose of LRH-A 220 micrograms to 30 micrograms and HCG 600 to 1000 international units per kilogram of female broodstock, halving the male dose. Female broodstock uses two pectoral fin injections. The first injection of 1/3 of the total dose, the interval between 14 hours to 18 hours for the second injection, use up the balance. The male fish was injected once at the second injection of the female.
3. Spawning and Incubation When the water temperature is between 24°C and 26°C after the second injection of the female parent fish, the effect time is 28 hours to 24 hours.
(1) Artificial insemination. Within 1 hour to 2 hours after the expected effect time, the egg is squeezed manually, and the male is killed by cutting and grinding the sperm, so that the egg is fully mixed, and after about 16 seconds, slowly adding a little water with the feathers constantly stirring , complete the fertilization process. After about 30 seconds, the fish eggs were glued to the brown tablets and hatched in the hatchery.
(2) Natural fertilization. Prior to the second injection of oxytocin in the female parent fish, a brood was placed in the spawning tank, which used sterilized brown tablets. After injecting oxytocic drugs, females and males were placed into the spawning tank at a ratio of 1:1.5. Within about 2 hours to 3 hours before the effect time is reached, flushing is used to stimulate estrus. When the effect time arrives, the flushing stimulation is changed to micro-flow stimulation, allowing it to spawn naturally, to finish sperm, and to complete the entire process in the spawning pool. Fertilization process.
(3) Incubation. Incubation is carried out in cement pools using microfluidic water incubation. Incubation water is sun-fired and gas-fired well water. The ground water meets the water quality regulations of Nr5051. The hatching pond covers an area of ​​5 to 10 square meters, with a water depth of 0.6 to 0.8 meters. The water temperature is controlled at 24 to 26 degrees Celsius. Ovulation density 3000 to 5000 tablets / square meter. During hatching, cover the sun above the hatchery to prevent direct sunlight from the fish eggs.
The fertilized eggs are incubated at 24°C~26°C, and the fish are normally hatched after 72 hours. The fish began to feed three days after they came out of the film. The open bait used zooplankton such as rotifers or Cladocera, copepods, etc., and can also be fed with filtered yolk. After about 10 days of incubation in the hatchery, the fry can swim normally and actively take in outside food before it can be cultivated.
The quality of the water source for cultivation of ponds shall comply with the provisions of GB 11607, and the water quality of the ponds shall meet the requirements of Nr 5051. The cultivation pond area is 0.5 mu to 1.0 mu, and the water depth is 0.5 meters to 0.8 meters. The ponds must be cleaned and disinfected prior to release. Method is: drain pool water dredging, sun 3 days to 5 days, then add water 10 cm to 15 cm, every 667 square meters of water with lime 150 kilograms watered into a slurry, spread evenly hot the whole pool, the wild fish Kill all. After 3 days, water is injected to 0.8 meters, the pH is stable at 7~8, and fish is released after fertilizing water quality. After the fish is fed into the pond, sufficient natural food can be eaten. Stocking density of 15,000 to 20 thousand tails is appropriate.
After the bait is fed to fish ponds in addition to fertilizing the water quality, it is also necessary to feed the sturgeon fish to eat and mix the fish and fish to wrap the fish to induce and acclimate. After one week, the fish and the wheat flour can be directly fed and mixed into the fish. The amount of daily feeding is controlled at 3% to 10% of the fish's body weight, and it should also be based on the weather, water temperature, and the fish's eating and feeding conditions. It should be fed twice daily. According to the significant photophobia of the stingray, the amount of feeding in the evening should account for 60% of the daily feeding amount, and the amount of feed should be within 2 hours of each feeding amount.
Daily management During the cultivation process, fresh water should be filled every 3 days to 5 days, each time filling 5 cm to 10 cm; regularly pouring lime slurry to make the pool water concentration reach 15ppm~20ppm, or every 15 days~20 Sprinkle bleach once a day, the concentration of 1ppm; adhere to the bait fish, food Taiwan, food industry disinfection, to ensure that the water quality is fresh, no disease. When the fry grows to more than 6 cm, it will be cultivated for about one month before entering the adult fish breeding stage.
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