Japanese Koi Artificial Propagation Technology

1. Broodstock selection

The characteristics of the varieties should be obvious: strong physique, bright body color, cloud-like spots, pure and no-nosed spots, stable genetic traits, gonads with well-developed gonads as reserve broodstock. The broodstock should be 3 years old or older, the carp weight should be 2.5kg or more, the carp weight should be 2kg or more, the carp ratio should be 1:1, but 1:2 should be preferred.

2. Intensive cultivation of broodstock

The broodstock should be stocked and raised. Usually, the water depth is 1.2m, and the cement pool with an area of ​​100m2 is raised. Each pond can be stocked with 4 to 8 tails. The microfluidic aquaculture requires the dissolved oxygen in the water to be 5mg/L or more. Water temperature 18 °C ~ 22 °C. Feed the squid buoyant bait, if necessary, with squid feed. The feed amount is calculated as 2% of the weight of the broodstock each day, and the feeds should be given once each afternoon.

3. Incubation hatching

(1) The condition of the urine production hatch pool can prepare 10 cement pools of 4m1.5m0.8m for use. The top of the pool should be covered with a shade net and plastic film to prevent acid rain and avoid glare. Set up a small water tower, use the micro-flow water after sand filtration, oxygenation of the inflator, three air stones per tank, depth control 0.6m, water temperature control at 22 °C ~ 24 °C. A 3.4m1.2m0.8m cage was placed in the pool, and four bamboo trusses were placed above the pool. Each bamboo basket was bred with a brown-fish nest that had been sterilized every 25cm.

(2) Pre-oxygenation inspection

At a water temperature of 21°C, it is possible to begin to examine the development of the gonadal gonads. If the gonad gonads are not yet developed, they can be cooked by LRH-A2, the squid is 0.3 μg/kg, the squid is halved, and the back muscles are injected. Good development of gonads does not require ripening. 8 days to 10 days after the re-examination, when the carp abdomen enlargement, obvious gonad outline, soft, reddish vent hole, light squeeze a small amount of eggs excreted, indicating that the gonad gonad development is mature, can be immediately transferred to oxytocin pool for oxytocin production.

(3) Oxygen production drugs and dosage

The oxytocin drug was LRH-A2, and the injected dose of carp was 1.5 μg/kg. The carp was halved and injected at the base of pectoral fins. After the injection, the broodstock is put into the urine production pool. Each pool is filled with 1 ♀ 1 ♂ or 1 ♀ 2 ♂, and the inflation and microfluidic water stimulation stimulate the production and the effect time is 21 43 43 hours.

(4) Spawning and fertilization Generally, at 4 am, carp starts to chase squid vigorously between fish nests. After more than half an hour, the oviposition and fertilization process is completed. Fish eggs adhere to the fish nest cage. The broodstock can be returned to the broodstock for restorative culture at dawn, and the individual broodstock is domesticated for the 2nd spawning. 3 to 4 instar Koi spawned 100,000 to 300,000 eggs at a time, and the fertilization rate was generally 80% to 90%.

(5) Hatching

Check the spawning situation. If there are many fertilized eggs, move the fish nests into other pools. Each pool is controlled at 50,000 to 100,000 grains, then Quanchiposa 1ppm methylene blue to prevent and control water mold. Fertilized eggs can be hatched from 76 hours to 92 hours at 22°C to 24°C. The hatching time is negatively correlated with water temperature.

(6) Holding

Newly hatched fry have poor swimming ability and are often attached to cages and fish nests. When the yolk sac disappears after 2 to 3 days, the digestive system is initially formed and the seedling enters the level of the leveling tour. At this time, food intake may be started. The saccharomyces algae can be fed, the 150-mesh sieved egg yolk, and the food-eating mother. Health, soy milk and so on. After feeding for 3 days to 5 days in the cage, the fry larvae inflate and the yolk sac completely disappears. When the fry have strong swimming and predation ability, they can send the pond.

4. Breeding

Seed cultivation and daily management are basically the same as carp. Seed pools can be used as old or old ponds, with an area of ​​400m2 to 800m2. The depth of the pool is about 1.2m. It is convenient for water injection and drainage, and the amount of sludge is required. Each tank is equipped with 1 to 2 aerators. 10 days before planting, use quicklime to disinfect 50kg/mu, then apply 400kg to 500kg/mu of fermented and sterilized pig manure (using chemical fertilizer), and then inject water to 50cm to feed the algae species. It will be medium-green with a large number of rotifers, horns, etc., to meet the needs of fry. Each acre can have 150,000 ponds, and every 100,000 fry can feed 2 kg soybean milk every day, which is divided into 2 feedings. As the fry grows, the water level increases, and the milk can increase gradually. Each day can also be fed with 1 carp bait, 100g to 400g each time. In this way, after about 20 days of raising, the fry can grow into fry fish fry about 3cm. At this time, it can be sold for the first time or grading.

5. The screening of fish fry is large and three-colored. Red-and-white koi grow to 60 days of age. When the body length is 8cm to 10cm, body color is obvious and basic stereotypes can be established. At this time, grade screening can be performed. Before net screening, 2 or 3 pull-net exercises are performed before screening, and then screening is performed to retain the inferior. Screening criteria: Retention of varieties with obvious characteristics, robust physique, slender body shape, bright color, color patches like clouds, perfect fish scales without spots is better. Obliterate deformity, dull color, poor growth, variegated individuals.

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