U.S. frogs are bred according to conventional methods and it is difficult to achieve a market size of more than 250 grams in the current year. To shorten the frog breeding cycle, the following measures can be taken. 1. Artificial indoor temperature-controlled culture is generally conducted in winter. Choose a thick, well-sealed, well-sized room for the frog house and a frog pool with a concrete structure. The area of ​​the pool is generally no more than 4 square meters, the pool is 0.8 meters high, the bottom is inclined at 15 degrees, and the pool can be counted side by side, each with an inlet and outlet. Before restocking, use a solution of 3ppm bleach (containing about 33% of available chlorine) to disinfect the pool, frog house and utensils, etc. After 72 hours, drain the pool water. The healthy, disease-free frogs were sterilized with 15 ppm potassium permanganate solution and 3% saline for 15 minutes and then stocked. Usually fed by artificial feed pellet feed, the daily feeding amount is 10% to 15% of the frog's weight. The room temperature is controlled above 20°C. Rinse once daily with water every morning and evening. Disinfect with 1ppm bleach or potassium permanganate once every two weeks. Regularly mix 100 kg of feed with 20 grams of oxytetracycline for 2 days. This method can make American frogs grow normally in winter, and the survival rate is as high as 95%. The disadvantage is that the investment is large. 2. Cultivation of greenhouses usually takes place during autumn and winter and late winter. Above the frog pool, take a 1.5m high plastic greenhouse. Pool width of 4 to 5 meters, a small pool in the middle for easy operation. Daily management work is basically the same as conventional farming. Before and after the winter, greenhouse cultivation was adopted to extend the frog's feeding time by about 2 months. The young frogs that had just become abnormal in autumn and winter could fully ingest before winter, which was beneficial to increase the survival rate of wintering; , May spawning 15 to 30 days in advance. 3. For artificial oxytocin production, healthy frogs with longer ancestry, longer body shape, and obvious sexual characteristics are generally required to weigh more than 400 grams. Chorionic gonadotropin (HCG) and luteinizing hormone-releasing hormone analogue (LRH-A) are commonly used for artificial labor. More than 500 grams of female frogs were injected with HCG 2000 plus LRH-A 200 micrograms of mixed drugs 1.5 to 2 milliliters and female frogs weighing less than 500 grams were injected with 1.5 milliliters, and the male frog dose was halved. At the 45-degree angle of the gluteal muscles, 1.5 cm of needles were injected. After the injection, the injection sites were rubbed with alcohol balls and carefully placed in the spawning pool at a ratio of 1:1. After 40 hours, the frog hugs to spawn. This method can advance the spawning period, spawning at the same time, and facilitate daily management. 4. Impulse electrical stimulation of early embryos Two hours after spawning, the fertilized eggs were placed in trays, treated with electrical pulses for 6 minutes, and then transferred to cages for hatching. In this way, the hatching rate can be increased by more than 10%, the worms can be metamorphosis 7-10 days earlier, and the frog's disease resistance can be enhanced and the growth rate can be increased. 5. Embed the cages in the earthen pond to hatch and raise the earthworms to adjust the earth's water level to about 50 cm. The length and width of the hatchery are all 1 meter long and 0.7 meters high. The bottom of the cage and the bottom of the cage are fixed with wooden stakes and the water is 30 centimeters. The fertilized eggs should be evenly distributed in the incubator and be floating. At the same time, attention should be paid to the timely removal of dead eggs. The hatching density should be less than 10,000 grains per square meter of water. Pool disinfection with earthen pond farming. Dianchi cages are usually filled with 40 cm of water, and the feeding amount is based on two hours of basic food. During the wintering period, the cages sink to the bottom of the pool. China Agricultural Network Editor
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