Virus acquisition and processing

First, the collection of venomous lice In the case of anger, due to the defense or attack instinct, the venom will be discharged from the sac. The collection of narcotics is based on this principle. The common methods for collecting scorpion venom include the trimming, artificial stimulation and electrokinetic methods.
(A) The tail-cutting method uses tweezers to clamp the sides of the fifth quarter of the scorpion's posterior abdomen. The tail section of the scorpion is cut with scissors in one hand. After being broken, it is immersed in physiological saline. The poisonous part is leached and the toxic liquid is placed. In the container, dry poison powder was again prepared and stored at -5°C until use. This kind of poisoning method can only collect poison once for each scorpion, and the scorpion after poisoning reduces the medicinal function and economic value, and the damage to the scorpion is too large, so usually this method is generally not used.
(b) artificial stimulation method is generally used tweezers clip a scorpion chelonian limb, lifted hanging in the container for a while, the majority of scorpion tail will discharge the venom, but there are a few scorpions do not drain venom, no venom can be fine Wooden chopsticks gently hit the head and chest or front abdomen of the scorpion, stimulating the tail thorn detoxification of the scorpion.
(3) Electrostimulation method 1 Equipment and equipment: 1 set of electronic virus-eliminating equipment and 1 wooden poisoning worktable (replaceable with a 2-drawer desk). Above the worktable, a 5-mm-thick glass plate with an area similar to that of the workbench was placed, and the virus-eliminating equipment was placed on the glass plate. One 60-ml beaker, one hair dryer, one watering can, one pair of latex gloves, a little talcum powder, one stainless steel needle and one sieve for 20 mesh (the inner wall of the sieve should be affixed with a wide transparent tape to make it Smooth to prevent escape of mules, specifications are: height 15 cm, length 60 cm, width 50 cm.
2 poisoning methods: beaker washed and disinfected, drained water, weighed and placed on the glass plate of the workbench, put a stainless steel needle in the beaker. The length of the needle should be slightly higher than the cup. Put the dumplings that are ready to be used for poisoning on the sieve for 2 hours. After the dumplings have wiped off the sediment and attachments on the body, use a hair dryer to blow off the dust from the carcasses. Then, spray a small amount of water on the carcasses with a watering can. The saline was used for poisoning. Workers should wear clean white work clothes and work caps during poison collection, rub a few talcum powders with hands and wear latex gloves. Then turn on the power of the electronic pulse instrument. Insert the two animal tweezers plugs with splices and place the foot switch on the floor near the right foot. Both hands picked up two scorpions, one on each side of the fifth quarter of the abdomen, and the other on the fifth section of the abdomen. The other scorpion caught the first quarter of the scorpion's tail, and the scorpions faced the belly downwards. The stingers faced the beaker and moved closer to the beaker. Inside, the foot switch is energized for 1-2 seconds, the lice will automatically discharge the venom, and immediately lift the foot to disconnect the power. If the venom of the stinger tip hangs, immediately wipe it on the needle in the beaker and wipe off the venom on the stinger.
3 Precautions
A. During the process of poison collection, strict attention should be paid to hygiene to ensure the purity and quality of the poison.
B. Some crickets do not detoxify during a single pass. They must be turned on again. However, the power-on time must be kept within 1-2 seconds and must not exceed 2 seconds to avoid burning the instrument and damaging the lice.
C. The amount of detoxification of the dumplings varies with the temperature. When the temperature is high, the amount of detoxification is relatively high. When the temperature is low, the amount of detoxification is relatively small. When the temperature is lower than 20° C., the detoxification amount of the dumplings is quite small, and some are almost in stagnation. When the temperature is below 10°C, the dumplings stop detoxification. Therefore, it is necessary to start collecting poison when temperature is higher than 25°C in June when the temperature-raised seedlings are used for breeding. When September temperatures gradually drop below 25°C, it is better to stop collecting poisons.
D. Pre-pregnancy maternal and pregnant lice cannot be used for poisoning. The dumplings used for poisoning are mostly commercial and old. However, it is better to use larger males that do not use for breeding, because males, individuals, and large platoons have the largest amount of 4-5 drops of detoxification.
Second, the initial processing of scorpion venom scorpion venom liquid is easily deteriorating at room temperature, and can only be stored in the refrigerator for about half a month. It must be processed into dry semolina before it can be stored for a long time. The processing method is as follows: liquid scorpion venom is first frozen in the refrigerator, and then transferred into a vacuum drier after freezing, and an appropriate amount of calcium oxide is used as a desiccant at the bottom of the drier. The desiccant is covered with four layers of new gauze and placed on the gauze. With a virulent beaker, followed by pumping with a vacuum pump, during the pumping process, attention should be paid to observation. If a large amount of air bubbles are found on the surface of the venomous poison, it is necessary to stop for a while and then pump until it is basically dry and let it rest for 24 hours. The scorpion is completely dried and then turned into granular crystals of different sizes. This is the crude dry powder that has been processed for the first time. Scrape the dry poison powder into small bottles, dissolve the wax and seal it, label it, indicate the preparation date and weight of the scorpion venom powder, and put the opaque black paper on it. Store in a refrigerator at -5°C for 5 years without deterioration. .

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